A method for generating highly multiplexed ChIP-seq libraries

By E. Ford, Chrysa Nikopoulou, Antonis Kokkalis, Dimitris Thanos. 2014

The barcoding of next generation sequencing libraries has become an essential part of the experimental design. Barcoding not only allows the sequencing of more than one sample per lane, but also reduces technical bias. However, current barcoding strategies impose significant limitations and/or technical barriers in their implementation for ChIP-sequencing. Converting Y-shaped sequencing adapters to double stranded DNA prior to agarose gel size selection reduces adapter dimer contamination and quantitating the number of cycles required for amplification of the library with qPCR prior to library amplification eliminates library over-amplification. We describe an efficient and cost effective method for making barcoded ChIP-seq libraries for sequencing on the Illumina platform.

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Citations in the corpus

• Structural Basis of Dual Specificity of Sinorhizobium meliloti Clr, a cAMP and cGMP Receptor Protein (2023, methodology): “The DNA library preparation was carried out as described previously (81) with the adapter sequences 2, 4 to 7, and 12 to 14 compatible with Illumina’s TruSeq platform.”