Three-dimensional fluorescent particle tracking at micron-scale using a single camera

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Particle imaging techniques for volumetric three-component (3D3C) velocity measurements in microfluidics (2011, methodology): “The threedimensional movement of coli bacteria in a volume of 481 9 335 9 65 lm3 was measured by Wu et al. (2005) using the defocused bacteria images with a conventional microscope and illumination by a mercury lamp.”
Microscale 3D flow mapping with μDDPIV (2007, background): “Wu et al. (2005) took advantage of the 3D diffraction ring patterns of small tracer particles when out-of-focus to track 3D particle motions.”