Functional Analysis of Cyclin-Dependent Kinase Inhibitors of Arabidopsis

By L. de Veylder, T. Beeckman, Gerrit T. S. Beemster, Luc Krols, Franky R. G. Terras, I. Landrieu, E. Van Der Schueren, S. Maes, M. Naudts, Dirk Inzé. The Plant Cell Online, 2001

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Cyclin-dependent kinase inhibitors, such as the mammalian p27Kip1 protein, regulate correct cell cycle progression and the integration of developmental signals with the core cell cycle machinery. These inhibitors have been described in plants, but their function remains unresolved. We have isolated seven genes from Arabidopsis that encode proteins with distant sequence homology with p27Kip1, designated Kip-related proteins (KRPs). The KRPs were characterized by their domain organization and transcript profiles. With the exception of KRP5, all presented the same cyclin-dependent kinase binding specificity. When overproduced, KRP2 dramatically inhibited cell cycle progression in leaf primordia cells without affecting the temporal pattern of cell division and differentiation. Mature transgenic leaves were serrated and consisted of enlarged cells. Although the ploidy levels in young leaves were unaffected, endoreduplication was suppressed in older leaves. We conclude that KRP2 exerts a plant growth inhibitory activity by reducing cell proliferation in leaves, but, in contrast to its mammalian counterparts, it may not control the timing of cell cycle exit and differentiation.

Subfields: Oncology, Plant Science

View Paper: https://www.semanticscholar.org/paper/025d86b229f258b2dac1e8726dcb5baefe7fe454

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Comprehending crystalline β-carotene accumulation by comparing engineered cell models and the natural carotenoid-rich system of citrus (2011, neutral): “The transcript abundance was normalized against peanut Ubiquitin (Luo et al. , 2005) transcript levels.”
Cytokinin-Deficient Transgenic Arabidopsis Plants Show Multiple Developmental Alterations Indicating Opposite Functions of Cytokinins in the Regulation of Shoot and Root Meristem Activity Article, publication date, and citation information can be found at www.plantcell.org/cgi/doi/10.1105/tpc.014928 (2003, background): “In these plants, cell cycle progression was limited and the uncoupling of cell growth from cell division resulted in an increase of the final cell size (De Veylder et al., 2001).”