Rapid quantitative detection of chytridiomycosis (Batrachochytrium dendrobatidis) in amphibian samples using real-time Taqman PCR assay.

By D. Boyle, Db B. Boyle, V. Olsen, Jat Morgan, A. Hyatt. Diseases of Aquatic Organisms, 2004

1,281 citations recorded by the source corpus.

Subfields: Global and Planetary Change, Microbiology, Nature and Landscape Conservation

View Paper: https://www.semanticscholar.org/paper/01acb4acf44f1688bc2689d498b90891eada060b

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Citations in the corpus

Batrachochytrium salamandrivorans sp. nov. causes lethal chytridiomycosis in amphibians (2013, background): “dendrobatidis as assessed by sampling the skin using cotton-tipped swabs and subsequent performing qPCR (10).”
Large‐scale seasonal variation in the prevalence and severity of chytridiomycosis (2006, methodology): “Swabs were analyzed for the presence of B. dendrobatidis using established quantitative (real-time) polymerase chain reaction (qPCR) techniques (Boyle et al., 2004) and the changes described by Kriger, Hero & Ashton (2006).”
Diversity and functions of microscopic fungi: a missing component in pelagic food webs (2010, neutral): “Amphibian Mixophyes fasciolatus Batrachochytrium dendrobatidis Boyle et al. (2004)”
INVESTIGATING THE POPULATION‐LEVEL EFFECTS OF CHYTRIDIOMYCOSIS: AN EMERGING INFECTIOUS DISEASE OF AMPHIBIANS (2005, background): “Recent developments in PCR (polymerase chain reaction) techniques for nonlethal detection of the disease (Boyle et al. 2004) has made proposal (a) possible this summer for the first time.”
Rapid enigmatic decline drives the fire salamander (Salamandra salamandra) to the edge of extinction in the Netherlands (2013, methodology): “…were conducted on 5 specimens (Ghent University), including macroscopic examination and (q)PCRs for the detection of Batrachochytrium dendrobatidis, ranavirus, Chlamydiales, herpes viruses and circovirus (Mao, Hedrick and Chichar, 1997; Boyle et al., 2004; Halami, 2007; Martel et al., 2012).”
Tadpole Mouthpart Depigmentation as an Accurate Indicator of Chytridiomycosis, an Emerging Disease of Amphibians (2006, methodology): “The validity of this assumption is supported by the fact that both standard and Real-time PCR assays for B. dendrobatidis are extremely sensitive, capable of detecting an amount of B. dendrobatidis DNA equivalent to that found in 1–10 zoospores (Annis et al., 2004; Boyle et al., 2004).”
Widespread historical presence of Batrachochytrium dendrobatidis in African pipid frogs (2010, methodology): “8S ribosomal DNA region of Bd, using an ABI Prism 7000 Sequence Detection System (Applied Biosystems, Foster City, CA, USA) (Boyle et al., 2004).”
Amphibian Conservation Action Plan (2007, background): “New molecular diagnostic tests have been developed, validated and are now available (Annis et al. 2004; Boyle et al. 2004), and biochemical studies of amphibian defenses against the pathogen have begun (Rollins-Smith et al.”
Symbiotic bacteria contribute to innate immune defenses of the threatened mountain yellow-legged frog, Rana muscosa (2007, methodology): “To assess whether frogs were infected with Bd, the skin was swabbed as described by Kriger et al. (2006), and real-time Taqman PCR was used to determine the intensity of infection (or the number of zoospore DNA equivalents) (Boyle et al., 2004).”
Social Immunity in Amphibians: Evidence for Vertical Transmission of Innate Defenses (2011, methodology): “After a 15 min bath to collect secreted peptides, frog skin was swabbed for detection of infection with B. dendrobatidis (according to Boyle et al. 2004, Hyatt et al. 2007).”
A century of Batrachochytrium dendrobatidis in Illinois amphibians (1888 1989) (2015, methodology): “We coded samples as positive if two of three runs showed exponential amplification before cycle 50 (Cheng et al., 2011), which are approximately 0.01 zoospore equivalents (Boyle et al., 2004).”
Upward range extension of Andean anurans and chytridiomycosis to extreme elevations in response to tropical deglaciation (2007, methodology): “The swabs were analyzed in triplicate by Taqman realtime PCR assay (Boyle et al., 2004), CSIRO Livestock Industries, Australian Animal Health Laboratory, Australia.”
Survivorship in Wild Frogs Infected with Chytridiomycosis (2006, methodology): “Swabs were analyzed for the presence of Batrachochytrium dendrobatidis using established quantitative (real-time) PCR techniques (Boyle et al., 2004), and employing the changes described by Kriger et al. (2006b).”
Disease dynamics vary spatially and temporally in a North American amphibian (2011, methodology): “Infection prevalence and intensity were determined using quantitative PCR (Boyle et al., 2004).”