The CRISPR tool kit for genome editing and beyond

By Mazhar Adli. Nature Communications, 2018

1,375 citations recorded by the source corpus.

CRISPR is becoming an indispensable tool in biological research. Once known as the bacterial immune system against invading viruses, the programmable capacity of the Cas9 enzyme is now revolutionizing diverse fields of medical research, biotechnology, and agriculture. CRISPR-Cas9 is no longer just a gene-editing tool; the application areas of catalytically impaired inactive Cas9, including gene regulation, epigenetic editing, chromatin engineering, and imaging, now exceed the gene-editing functionality of WT Cas9. Here, we will present a brief history of gene-editing tools and describe the wide range of CRISPR-based genome-targeting tools. We will conclude with future directions and the broader impact of CRISPR technologies.CRISPR has rapidly become an indispensable tool for biological research. Here Mazhar Adli reviews the current toolbox for editing and manipulating the genome and looks toward future developments in this fast moving field.

Subfields: Insect Science, Molecular Biology, Public Health, Environmental and Occupational Health

View Paper: https://www.semanticscholar.org/paper/00a5ad8490128eb368004679b482abb69f90ebb3

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Molecular Biology

1. Adam Officer
2. Erick Armingol
3. Olivier Harismendy
4. Nathan E. Lewis

Citations in the corpus

• Deciphering cell–cell interactions and communication from gene expression (2020, background): “Particularly, removing or adding communication pathways with genome editing and cell engineering technologies 157 will modify cellular phenotypes to control how pairs of cells interact.”